[ Objective] The research aimed to explore the optimum extraction conditions of leaf protein from alfalfa. [ Method] Different material-water ratio, hearing temperature, flocculation time and pH were set up to study their effects on the extraction of leaf protein. [ Result ] When the material-water ratio was 1:4, heating temperature was 70℃, flocculation time was 9 min and pH was 5.0, the yield of alfalfa leaf protein and the extraction rate of crude protein were both the highest. [ Conclusion] The research could provide theoretical basis for the development and utilization of leaf protein.%[目的]探索苜蓿叶蛋白的最佳提取条件.[方法]设置了不同料水比、加热温度、絮凝时间、pH,研究其对苗蓿叶蛋白提取的影响.[结果]当料水比为1:4,加热温度为70℃,絮凝时间为9 min,pH为5.0时,苜蓿叶蛋白得率和粗蛋白提取率均最高.[结论]该研究可为叶蛋白的开发与利用提供理论依据.
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