贵州不同产地苗药飞龙掌血中总生物碱和白屈菜红碱的含量测定

摘要

[目的]测定贵州不同产地苗药飞龙掌血中总生物碱和白屈菜红碱的含量.[方法]采用反滴定法测定总生物碱含量,并采用高效液相色谱法测定白屈菜红碱的含量,色谱柱为Diamonsil C18(250 mm×4.6 mm,5 μm),流动相为乙腈-浓度0.1％磷酸水溶液(V/V,28∶ 72),检测波长为270 nm,流速为1.0 ml/min.[结果]总生物碱、白屈莱红碱的平均回收率分别为97.5％和99.8％；RSD分别为2.71％和0.40％.[结论]该分析方法准确、灵敏、可靠,可用于飞龙掌血中总生物碱和白屈菜红碱的含量测定.%[Objective] To determine the contents of total alkaloid and chelerythrine in Toddalia asiatica from different places in Guizhou. [Method] Total alkaloid was determined by back-titrantion method, and chelerythrine was determined by HPLC method, using Diamonsil C18 column (250 mm×4.6 mm,5 μn) , and acetonitrile -0.1% phosphoric acid ( V/V, 28:72) as mobile phase, with the detection wavelength at 270 nm and flow rate of 1.0 ml/min. [ Result] The average recoveries of total alkaloid and chelerythrine were 97. 5% and 99 8% with RSD = 2.71% and 0.40% respectively. [Conclusion] This analysis method is accurate, sensitive and reliable to determine the contents of alkaloid in Toddalia asiatica.