[Objective]The research aimed to investigate the effects of combined treatment of Sr2+ and Ca2+ on antioxidant enzymes activities of oilseed rape seedlings.[Method]Three-leaf stage oilseed rape seedlings ( Brassica napus L. cv. Mianyou No. 15) were raised in quartz-hy-droponic culture system, and a solution containing 0, 10, 20 or 40 mmol/L SrCl2, and 0, 5 or 10 mmol/L Ca ( NO,) 2 was applied. Three antioxidant enzymes (POD, CAT and SOD) activities were determined after 0, 7, 14 and 21 d of the combined treatment of Sr2* and Ca2*.[Result]POD, CAT and SOD activities were increased gradually with the increase of Ca2* concentration. Ca2* could alleviate the deleterious effect induced by Sr2* in oilseed rape seedlings.[Conclusion]The strontium accumulation capability of oilseed rape was explained from the perspective of antioxidant rapeseed, providing a theoretical basis for the use of oilseed rape phytoremediation of strontium to cleanup the polluted environments.%[目的]为了了解环境介质中同时存在Ca2+和Sr2+2种离子时油菜幼苗的抗氧化酶活性的变化.[方法]在石英砂和Hoagland营养液培养体系中,用浓度为0、10、20和40 mmol/L SrCl2及浓度为0、5和10 mmol/L Ca(NO3)2处理3叶龄油菜幼苗.在处理0、7、14和21 d后,分别取样测定POD、CAT和SOD的活性.[结果]在相同的Sr2+浓度下,随着Ca2+浓度的增加,POD活性增加,Ca2+明显缓解Sr2+对油菜幼苗的毒害作用.[结论]该研究从抗氧化角度初步阐明油菜具有较高富集Sr2+能力的机理,为利用油菜对锶污染环境进行植物修复提供理论依据.
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