蛋白质组学技术是21世纪研究生命科学最有价值的技术之一,将其应用到精子和精浆蛋白质的研究后,已取得了许多成果.对蛋白质组、蛋白质组学、双向电泳、质谱、生物信息学等进行了简要介绍,并重点阐述了蛋白质组学技术在精子蛋白和精浆蛋白上的研究进展.%[Objective]The research aimed to optimize laboratory artificial rearing methods for Plutella xylostella L. and to select out effective insecticides against this pest species.[Method]Brassica chinensis seedlings were cultivated in the laboratory at (25 ±1 )℃ , 60 -75% RH, and a photoperiod of 14:10 ( L:D) h. The green blow molding papers were used to collect eggs, and Brassica oleracea seedlings were cultivated to breed Plutella xylostella L. until the insects were ready for use. Meanwhile, the susceptibility of 3rd instar larvae of Plutella xylostella L. to five types of insecticides was tested by leaf-dipping method. [Result]Plutella xylostella L. larvae were found well-grown and uniform in devel opmental stages with average pupa weight of 5.4 mg, pupa emergence percentage of 94.0 % and egg number of 135. 1 per female. The LC50, values of 3rd instar Plutella xylostella L. larvae against 200 g/L chlorantraniliprole SC, 20% flubendiamide WG, 150 g/L indoxacarb EC, 200 g/L tebufenozide SC and 1.8% abamectinEC, were 0.152, 0.223, 1.151, 93.340 and 0. 128 mg/L, respectively. [Conclusion]This rearing method is easy to be implemented and of great practicality, suitable for mass propagation of P. xylostella L. Abamectin, chlorantranil iprole and flubendiamide showed high insecticidal activities, so these three insecticides can be used alternately for the field prevention and con trol of Plutella xylostella L.
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