[目的]解析紫花苜蓿、黄花苜蓿和胡卢巴属植物的亲缘关系.[方法]利用ISSR分子标记技术,对紫花苜蓿、黄花苜蓿和胡卢巴种质资源之间的亲缘关系进行研究.[结果]紫花苜蓿、扁蓿豆和黄花苜蓿有较广遗传基础,胡卢巴种质材料相对于紫花苜蓿种质材料与黄花苜蓿的亲缘关系更近.苜蓿属和胡卢巴属亲缘关系研究有利于对一些存在争议的中间过渡类型进行分类,但在对中间过渡类型分类时应找一个合适的“度”,以鉴定存在争议的中间过渡类型属于胡卢巴属还是苜蓿属.[结论]该研究结果为一些植物材料中间过渡类型的分类学研究提供了指导.%[Objective]To establish B. falcatum callus rapid propagation system.[Method]With the reference to the average growth of cells, the media, initial pH value, 6-BA, NAA and 2,4-D of B. fakatum callus were optimized by orthogonal optimal design method.[Result]The five factors had significant influence on the test results, especially N6, the initial pH of 5.6 was conducive to the growth of cells; of the three hormones, the largest cell fresh weight can be obtained when the combination of 6-BA (2 mg/L) and NAA (0. 5 mg/L) was added to the medium. The most advantageous medium for B. falcatum callus cell growth was N6 +2 mg/L 6-BA + 0. 5 mg/L NAA, pH 5.6. The cell growth of B. falcatum callus was inhibited by 2,4-D when it was added in medium.[Conclusion]The study results provide experimental data and theoretical support for further expanding the culture and establishing the suspension system of B. falcattan callus.
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