[Objective] To determine the sensitivity of calf diarrhea E. coli !o common disinfectants in order to provide a theoretical basis for the correct use of disinfectant in dairy farm. [Method ] The minimum inhibitory concentration, minimum bactericidal concentration, and quantitative bactericidal rate of Benzalkonium chloride, Omnicide and Trichloroisocyanuric acid to calf diarrhea E. coli from Shihezi region were determined. [Result] The best inhibitory concentration of omnicide, trichloroisocyanuric acid and benzalkonium chloride were 1:240, 1:1 200 and 1:1 200 respectively, the minimum bactericidal concentration were 1:80,1:600 and 1:800, the quantitative bactericidal rate against E. coli were 99. 86% -99.88% by trichloroisocyanuric acid(1:1 000-1 600), 99.12% -99.24% by omnicide (1:160-240) and 99. 86% -99- 88% by benzalkonium chlonde( 1:1 000 - 1 600) at the recommended concentration respectively. [Conclusion] Calf diarrhea E. coli was sensitive to trichloroisocyanuric acid, followed by omnicide and benzalkonium chloride.%[目的]测定犊牛腹泻大肠杆菌对常用消毒剂的敏感性,为牛场消毒剂的合理使用提供理论依据.[方法]选用常用的拜洁、安灭杀、三氯异氰尿酸3种消毒剂,对来自石河子地区不同牛场犊牛腹泻大肠杆菌分离株进行了最小抑菌浓度、最小杀菌浓度以及定量杀菌率的测定.[结果]安灭杀、三氯异氰尿酸和拜洁的最佳抑菌浓度分别为1∶240、1∶1 200和1∶1 200.5 min内不同浓度的3种消毒剂对10株大肠杆菌菌株的最小杀菌浓度分别为1∶80、1∶600、1∶800.在推荐浓度下,对石河子地区犊牛腹泻大肠杆菌,三氯异氰尿酸(1∶1000～1600)杀菌率为99.86％ ～ 99.88％,安灭杀(1∶160 ～240)杀菌率为99.12％ ～ 99.24％,拜洁(1∶1 000 -1 600)杀菌率为97.64％～98.36％.[结论]犊牛腹泻大肠杆菌对三氯异氰尿酸最为敏感,而安灭杀和苯扎氯铵次之.
展开▼
