流感病毒快速诊断试剂中HA抗原热稳定性的研究

摘要

[Objective] The research aimed to screen out the protective agenl of influenza virus. [Method] H3N2 and H1N1 subtypes of influenza vims were added to A - F groups to make the accelerated teat at 37 ℃. And HA tiler was test by hemagglutination test. [ Result] HA antigen hemagglutination liter of H3N2 and HIN subtype of influenza virus in A group (adding PBS buffer)were 20 and 32 respectively after 28 days. The heat stability of HA antigen in F group (adding BSA.fucose.Proclin 300,Triton 100) were the best and HA hemagglutination titer of H3N2 and H1NI subtype of influenza virus were 48 and 96 respectively after 28 days. [Conclusion] The components in F group had the best protective effects on HA antigen of influenza virus and could be used as the candidate agent of protective agent.%[目的]筛选流感病毒HA抗原保护剂.[方法]将H3N2和H1N1亚型流感病毒分别加入A～F组中,在37℃下进行加速试验,并通过血凝试验测定HA滴度.[结果]在28dA组(添加PBS缓冲液)中H3N2和H1N1亚型流感病毒的HA抗原血凝滴度分别为20和32.F组(添加BSA、岩藻糖、Proclin 300 、Triton 100)HA抗原的热稳定性最好,在28 d H3N2和H1N1亚型流感病毒HA抗原血凝滴度分别为48和96.[结论]F组成分对流感病毒HA抗原的保护作用最好,可作为保护剂候选配方.