青钱柳愈伤组织诱导与增殖的初步研究

摘要

[Objective] To carry out preliminary study on callus induction and proliferation of Cylocarya paliurus. [Method] The leaf and stem segments of Cylocarya paliurus as explants were cultured on MS, WPM and improved DKW3 mediums, added 6-BA and IBA with different concentration ratios to explore the optimum medium for callus induction and proliferation. [ Result] It was found that MS +4.0 mg/L 6-BA + 2.0 mg/L IBA was the optimum medium for inducing stem callus of Cylocarya paliurus, MS +2.0 mg/L 6-BA +0.5 mg/L IBA was the optimum medium for inducing leaf explants callus of Cylocarya paliurus, and MS + 1.0 mg/L 6-BA + 1.0 mg/L IBA was the optimum medium for callus proliferation. 200 mg/L Vitamin C was best to inhibit Cylocarya paliurus callus from browning with the browning rate of only 5. 71%. [ Conclusion ] This study provided some theoretical basis for the establishment of tissue culture and rapid propagation system and the development of molecular breeding study of Cylocarya paliurus.%[目的]对青钱柳愈伤组织的诱导与增殖进行初步研究.[方法]以青钱柳茎段和叶片为外植体,利用MS、WPM、改良DKW 3种基本培养基,添加不同浓度配比的6-BA和IBA,来探讨愈伤组织诱导和增殖的最佳培养基配方.[结果]诱导茎段和叶片愈伤组织产生的最佳培养基配方分别为:MS +4.0 mg/L 6-BA+ 2.0 mg/L IBA和MS +2.0 mg/L 6-BA +0.5 mg/L IBA；愈伤组织增殖时的最佳培养基为:MS+1.0 mg/L 6-BA+1.0mg/L IBA.200 mg/L Vc对抑制青钱柳愈伤组织褐化效果最好,褐化率仅为5.71％.[结论]为青钱柳组培快繁体系的建立和分子育种研究的开展奠定了一定理论基础.