HPLC法测定黄芩毛状根转化产物熊果苷的研究

摘要

[目的]建立高效液相色谱法( HPLC)测定黄芩毛状根中熊果苷及残余底物对苯二酚含量的方法.[方法]采用乙醇回流法提取加底物转化后的毛状根,并采用HPIC法测定黄芩毛状根中熊果苷及残余底物对苯二酚的含量.色谱条件:色谱柱为Symmetry-C18(250mm×4.6 mm,5 μm),流动相为无水甲醇-水(15:85,V/V),流速为0.8 ml/min,检测波长为287 nm.[结果]在所用流动相条件下,熊果苷及对苯二酚峰型和分离效果良好,熊果苷标准曲线线性范围为0.100 ～0.200 mg/ml(R =0.999 1),精密度RSD为1.52％,回收率为98.6％ ～ 100.3％.样品中熊果苷的含量为45.5 mg/g；以外标一点法测得毛状根中残留对苯二酚的含量为0.22 mg/g.[结论]该方法准确可靠,可用于黄芩毛状根转化产物熊果苷及残余底物对苯二酚的含量测定.%[Objective] The research aimed to build high-performance liquid chromatography (HPLC) determination method to determine arbutin in Scutellaria baicalensis hairy roots and residual substrate hydroquinone content. [Method] Using ethanol refluxing method,extract hairy roots from converted substrates. And using HPIC method,determine arbutin and residual substrate hydroquinone content in Scutellaria baicalensis hairy roots. The chromatographic conditions were:chromatographic column:Symmetry-C18(250 mm ×4.6 mm,5 μm),mobile phase was absolute metha-nol-water (15:85, V/V) ,flow rate of 0. 8 ml/min,detection wavelength was 287 nm. [Result] Under the mobile phase conditions,arbutin and hydroquinone has a good peak shape and separation effect. The standard linear range of arbutin was 0. 100 -0.200 mg/ml (R =0.9991),precision RSD was 1.52% ,recovery rate was 98.6% -100.3%. [Conclusion] This method could accurately measure the content of Scutellaria baicalensis hairy roots transformation arbutin and residual substrate hydroquinone content.