微切变－助剂互作技术制备鹿角盘微切助粉及其活性物质的检测

摘要

[目的]检测鹿角盘微切助粉中与骨质疏松症有关的活性物质的含量。[方法]采用微切变－助剂互作技术制备鹿角盘微切助粉，通过与中药粉碎技术相比，考察该技术对鹿角盘颗粒粒径、形态的影响并检测其中活性成分的含量。[结果]经微切变－助剂互作技术处理后，鹿角盘粒径在1～30μm范围内的颗粒数占82．73％，且细胞已被充分破碎，有效成分呈释放的状态。鹿角盘微切助粉中含有丰富的性激素（包括雌二醇、睾酮、孕酮）、类胰岛素样生长因子－1、柠檬酸钙和钙，且含量均高于其粗粉组，说明微切变－助剂互作技术有利于鹿角盘中活性成分的释放，体现了该技术的优越性。[结论]该研究可为合理开发利用鹿角盘提供一种新的粉碎技术，并为阐明鹿角盘防治骨质疏松症提供理论依据。%Objective] To detect the active ingredients content which related to osteoporosis in deer antler base PAI powder processed by press-shear assisted interaction technology (PAI).[Method]We first used press-shear assisted interaction technology and traditional Chinese medicine grinding technology to process deer antler base.Effects on particle sizes, micrographs and active ingredients content by different pre-treatments were also investigated.[ Result] Particle number of deer antler base PAI powder ranged in 1-30 μm accounted for 82.73%.Cells were broken and the active ingredients were released.Deer antler base PAI powder contained many sex hormone ( including estradiol, testos-terone, progesterone), IGF-1, calcium citrate and calcium, and the content of several active ingredients in deer antler base PAI powder were superior to its coarse powder.Above results indicated that press-shear assisted interaction technology was beneficial to the release of the active ingredients.[Conclusion] This research could provide a new grinding technology to promote the rational development and utilization of deer antler base, and supply a scientific basis for deer antler base applied for prevention and treatment of osteoporosis.