[目的]研究适合北五味子“红珍珠”组织培养的方法。[方法]以北五味子“红珍珠”为试材,研究不同外植体、激素对其组织培养诱导、分化和生根的影响。[结果]以春季嫩枝为外植体,分化培养基为1/2MS +6-BA 2.0 mg/L +NAA 0.01 mg/L +ZT 0.1 mg/L,以1/2MS+IAA 1.0 mg/L+IBA 2.0 mg/L 生根效果较好,有利于北五味子“红珍珠”根系的生长。[结论]筛选出北五味子“红珍珠”适宜的组织培养配方,为其大规模工厂化育苗提供技术支撑。%Objective] The aim was to obtain the suitable tissue culture method for Schisandra chinensis‘Red pearl’ .[ Method] The effects of different explants and hormones on the induction, differentiation and rooting of tissue culture were studied.[ Result] With spring shoots as explants, the differentiation culture medium was 1/2MS +6-BA 2.0 mg/L +NAA 0.01 mg/L +ZT 0.1 mg/L.1/2MS+IAA 1.0 mg/L+IBA 2.0 mg/L had better rooting effect, which was conducive to the growth of root system of Schisandra chinensis‘Red pearl’.[Conclu-sion] The suitable tissue culture formula of Schisandra chinensis‘Red pearl’ is selected, which can provide the technical support for large-scale breeding.
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