聚乙烯醇降解菌HK1菌株鉴定与降解特性

摘要

[Objective] The aim was to identify a polyvinyl alcohol degradable bacteria HK1 and study its degradation character.[Method] We took a strain HK1 which could grow with polyvinyl alcohol as the only carbon source,identified it through its morphology and physiochemical characteristics,studied the resistance to several kinds of antibiotics which were commonly used in the laboratory,and studied its degradation character through shake flask test.[Result] According to its morphology,physiochemical characteristics and 16S rDNA sequence analysis,HK1 was identified as Bacillus amyloliquefaciens.It could degrade 36.26% of PVA (1 g/L) after culturing at 30 ℃,180 r/min in shaking flasks.The optimal conditions for PVA biodegradation by the obtained strain were as followed:30 ℃,1 g/L PVA,pH 8.0,and 2 g/L yeast.[Conclusion] The results can provide reference for further research of the strain.%[目的]鉴定聚乙烯醇降解菌HK1并研究其降解特性.[方法]以一株能以聚乙烯醇为唯一碳源生长的细菌HK1为出发菌株,通过形态观察和生理生化试验对其进行鉴定,研究其对几种实验室常用抗生素的抗性,并通过摇瓶试验研究其降解特性.[结果]经鉴定,该菌确定为解淀粉芽孢杆菌属细菌,30 ℃、180 r/min摇床培养后,对培养基中PVA(1 g/L)的降解率达36.26%.HK1降解PVA的环境最优条件如下:温度为30 ℃,底物浓度为1 g/L,pH 8.0,酵母粉添加量为2 g/L.[结论]研究结果为系统研究该菌株提供了参考.