蓖麻RcAKT1基因的克隆与序列分析

摘要

[Objective]To clone and analyze RcAKT1 gene sequence from castor.[Method]The total RNA of castor fresh leaves was extracted and the RACE method was used to clone the 3 ′end and 5′ end of RcAKT1 gene.The two-terminal sequence was spliced by bioinformatics software DNAman.Finally,the ORF full length sequence of RcAKT1 gene was obtained and the bioinformatics analysis was carried out.[Result]The length of the open reading frame was 2 253 bp,the analysis predicted that 751 amino acids could be encoded without interruption.The AKT1 sequence of other plants was compared with the RcAKT1 sequence of castor.The results showed that the homology was high and the consistency was between 84% and 97%.[Conclusion]After bioinformatics analysis,it has been found that castor RcAKT1 belongs to the ANK super family and it''s a hydrophilic protein.%[目的]对蓖麻RcAKT1基因进行克隆和序列分析.[方法]提取盐胁迫处理的蓖麻新鲜叶片总RNA,采取RACE法克隆蓖麻RcAKT1基因的3′末端和5′末端,利用生物信息学软件DNAman对两端序列进行拼接,最后设计一对特异性引物,从而获得蓖麻RcAKT1基因的ORF全长序列,并对该序列进行生物信息学分析.[结果]该基因的开放阅读框序列长度为2 253 bp,推测可不间断编码751个氨基酸.选取其他植物的AKT1序列与蓖麻RcAKT1序列进行比对,结果显示同源性很高,其一致性在84%~97%.[结论]经过生物信息学分析,发现蓖麻RcAKT1属于ANK超级家族,是亲水性蛋白质.