蓼蓝组织培养再生体系的建立

摘要

[目的]建立蓼蓝组织培养再生体系.[方法]以半野生蓼蓝种子、幼叶和茎段为外植体,探讨不同浓度植物激素配比的培养基对其愈伤组织诱导、继代、分化及生根的影响.[结果]以蓼蓝茎段为外植体进行组培是适宜的;诱导和继代最佳培养基为MS+6-BA 1.0 mg/L+2,4-D 2.0 mg/L,平均诱导率可达73.33%;分化最佳培养基为6-BA 1.0 mg/L +NAA 0.5 mg/L,平均分化率可达51.67%;生根适宜的培养基为1/2 MS+NAA 0.5 mg/L,生根后移栽成活率达100%.[结论]该研究为优良蓼蓝快速繁殖提供了新的途径,并为其种质资源的保存与利用提供了依据.%[Objective]To establish regeneration system for tissue culture of Polygonum tinctorium. [Method]Its seeds, young leaves and stems were used as explants to explore influences of media with different concentration hormone on callus induction, subculture, differentiation and rooting. [Result]Stems of P. tinctorium were ideal explants. The optimum medium for callus induction and subculture was MS+6-BA 1.0 mg/L+2,4-D 2.0 mg/L, the corresponding average induction rate was 73.33%. The optimum medium for callus differentiation was 6-BA 1.0 mg/L +NAA 0.5 mg/L, the corresponding average differentiation rate was 51.67%. The optimum medium for seedlings rooting was 1/2 MS+NAA 0.5 mg/L. The surviving rate after transplanting attained to 100%. [Conclusion]The study provided a new way for P. tinctorium rapid reproduction,andprovided theoretical basis for the preservation and utilization of germplasm resources.