The effect factors on regeneration system of tissue culture using mature embryo of wheatgrass

摘要

It is the development trend that breeds new variety with good quality, high yield and multiresistance by transgenic technology in modern wheatgrass breeding.It is precondition of transgene that found a highly‐effect and stabilized regeneration system for tissue culture.Through the experiment on tissue culture using mature embryo of differentials genotype wheatgrass to select excellent genotype of wheatgrass that was suitable for transform exogenous gene.The effect factors of callus induction and regenerated frequency were studied in the regeneration system.