木橑尺蠖的不同虫态过冷却点测定

摘要

[目的]测定木橑尺蠖(Culcula panterinaria)各虫态的过冷却点扣结冰点,明确各虫态的耐寒性.[方法]采用数字万用电表和低温培养箱测定木橑尺蠖1～6龄幼虫、蛹和雌雄成虫的过冷却点和结冰点.[结果]木橑尺蠖的不同发育阶段过冷却点和结冰点大小顺序为1龄幼虫[(-10.01±0.24)、(-9.75±0.13)℃]、2龄幼虫[(-11.24±0.15)、(-10.89±0.16)℃]、3龄幼虫[(-12.64±0.23)、(-12.01±0.24)℃]、成虫[(-14.39±0.20)、(-13.76±0.22)℃]、4龄幼虫[(-14.56±0.15)、(-13.94±0.21)℃]、5龄幼虫[(-15.36±0.17)、(-14.85±0.18)℃]、6龄幼虫[(-17.87±0.13)、(-16.93±0.17)℃]和蛹[(-20.95 ±.0.09)、(-19.78±0.19)℃].木橑尺蠖的雌成虫过冷却点和冰点都低于雄成虫,木橑尺蠖雌成虫的过冷却点和结冰点分别为(-14.56±0.18)、(-14.61±0.19)℃,雄成虫分别为(-14.22±0.22)、(-12.91±0.25)℃.[结论]木橑尺蠖的不同发育阶段过冷却点和结冰点均存在显著差异,雌、雄成虫的过冷却点差异不显著,而结冰点差异显著.%[Objective] The aim was to examine super-cooling point and freezing point of Culcula panterinaria at different stages and clear cold tolerance of each insect state.[Method] The super-cooling points and freezing points at each development stage of C.panterinaria was measured by digital multimeter and low temperature incubator.[Result] The sequence of super-cooling point and freezing points at each development stage of C.panterinaria from high to low was the 1 st instar larvae [(-10.01 ± 0.24),(-9.75 ± 0.13) ℃],the 2nd instar larvae [(-11.24 ± 0.15),(-10.89±0.16) ℃],the 3rd instar larvae[(-12.64 ±0.23),(-12.01 ± 0.24) ℃],adult[(-14.39±0.20),(-13.76±0.22) ℃℃],the 4th instar larvae [(-14.56 ± 0.15),(-13.94 ± 0.21) ℃℃],the 5th instar larvae [(-15.36 ± 0.17),(-14.85 ± 0.18) ℃],the 6th instar larvae [(-17.87 ± 0.13),(-16.93 ± 0.17) ℃] and pupa [(-20.95 ± 0.09),(-19.78 ± 0.19) ℃℃].The super-cooling point and freezing point of C.panterinaria's female adult(-14.56 ±0.18),(-14.61 ±0.19) ℃ were lower than male adult(-14.22 ±0.22),(-12.91 ± 0.25) ℃.[Conclusion] There is significant difference of the super-cooling point and the freezing point for the C.panterinaria.And the difference of adult which were in different of super-cooling point is significant while the difference of freezing point is not.