引种成都后欧李生长期多酚含量的变化

摘要

[Objective] To explore changes of polyphenol content of Prunus humilis Bunge introduced in Chengdu during growing period and provide theoretical basis for researching polyphenol content of Prunus humilis Bunge.[Method] We determined the polyphenol content of Prunus humilis Bunge introduced in Chengdu during growing period by tartaric acid iron assay.[Result] The polyphenol of Prunus humilis Bunge extracted by using 95% methanol was the best,and the content of polyphenol of Prunus humilis Bunge would not accumulate internally in plants.The content of polyphenol reached higher in around 12:00 and when the temperature was higher in the evening,it would be the minimum at 14:00 when the temperature was the lowest,and the content was also very low in about 19 ℃;the content of polyphenol would increase under herbivore stress.[Conclusion] Polyphenol has important effect on metabolism of plant.The content of its fresh leaves of plants can reflect the intensity of secondary metabolism,the reaction of plant adaptation to the environment,and resistance to various environmental stresses.%[目的]探究欧李引种成都后其生长期多酚含量的变化,为欧李多酚类物质的研究提供参考.[方法]采用酒石酸铁比色法测定欧李引种成都后的多酚含量.[结果]用95%甲醇提取欧李多酚效果最好,欧李体内的多酚含量并不会在植物体内大量积累,多酚含量在12:00左右温度较高和傍晚时出现较大值,在14:00温度最高时较低,在19 ℃左右时也较低,当有虫害胁迫时欧李体内多酚含量增加.[结论]多酚对于植物的次生代谢有重要影响,在鲜叶中的含量可以反映植物次生代谢的强度,并反映植物对环境的适应性以及对各种环境胁迫的抵抗力.