野生大豆与栽培大豆杂交F1代ISSR多态性分析

摘要

[Objective] To sudy the difference of genetic polymorphism between Glycine soja and Glycine max,so as to provide theory evidence for Glycine max crossbreeding.[Method] Twenty primers were used for ISSR analysis on Glycine soja,Glycine max and 10 F1 hybrids,to study the genetic relationship of Glycine soja and Glycine max.[Result] Total 167 bands were amplified in the PCR reactions.Among them 121 were polymorphic bands and the polymorphic rate was 72.45%.The average genetic coefficients between Zhonghuang 19 and F1 progenies,ZYD04350 and F1 progenies were 0.65 and 0.47,respectively.[Conclusion]There was abundant genetic variation among F1 progenies derived from crossing between Glycine soja and Glycine max,genetic material of F1 hybrids obtained from maternal was more than male parents.%[目的]探究野生大豆与栽培大豆之间遗传多态性的差异,以期为大豆杂交育种的亲本选配提供理论依据. [方法]用20对ISSR引物对野生大豆与栽培大豆及其10份杂种F1材料进行遗传多样性分析,探讨野生大豆与栽培大豆之间的遗传关系. [结果]此次试验共扩增出167条带,其中121条为多态性带,多态率达72.45 %;杂交F1代材料与其母本中黄19及父本ZYD04350间的平均遗传相似系数分别为0.65和0.47. [结论]栽培大豆与野生大豆杂交F1代材料间存在着丰富的变异,杂交F1代从母本获得的遗传物质多于父本.