R318的抗褐飞虱特性与育种利用

摘要

[目的]研究R318的抗褐飞虱特性与育种利用.[方法]以TN1、RHT、B5为对照,对R318及其衍生品系进行褐飞虱田间抗虫鉴定,用抗性基因Bph15引物MS5对R318及其衍生品系进行分子检测.[结果]田间鉴定R318对褐飞虱表现为抗,其衍生品系表现为中抗-感虫;分子检测R318及其衍生品系与B5具有一致的带型.[结论]R318含有的抗性基因可能是Bph15.%[Objective]Characteristics of resistance to brown planthopper and breeding utilization of R318 were studied.[Method]With TN1, RHT and B5 as control, resistances to brown planthopper in field of the R318 and its derived strains were identified.Molecular detection of R318 and its derived strains was done by primer MS5 of resistance gene Bph15.[Result] R318 was manifested as resistance to brown plan-thopper by field identification, its derived strains were manifested as medium resistance-susceptibility to brown planthopper.R318 and its de-rived strains have indistinguishable fingerprinting patterns with B5 by molecular identification.[ Conclusion] It is considered R318 and its de-rived strains may contain resistance genes Bph15.