重铬酸钾和3,5-二氯苯酚对羊角月牙藻和斜生栅藻的毒性效应

摘要

[Objective] To study toxic effects of potassium bichromate and 3,5-dichlorophenol on growth of Selenastrum capricornutum and Scenedesmus obliquus.[Method] Growth inhibition test of potassium bichromate and 3,5-dichlorophenol was conducted with Selenastrum capri-cornutum and Scenedesmus obliquus over the 72-hour test period.[ Result] Based on the results, the 72-hour EyC50 of Potassium bichromate to Selenastrum capricornutum was 1.02 mg/L and 95% confidence limit was 0.98 -1.06 mg/L.The 72-hour ErC50 was 2.48 mg/L and 95%confidence limit was 2.25-2.74 mg/L.The 72-hour EyC50 of 3, 5-dichlorophenol to Selenastrum capricornutum was 1.12 mg/L and 95%confidence limit was 1.08-1.16 mg/L.The 72-hour ErC50 was 2.41 mg/L and 95% confidence limit was 2.22-2.62 mg/L.The 72-hour EyC50 of Potassium bichromate to Scenedesmus obliquus was 0.426 mg/L and 95% confidence limit was 0.418 -0.434 mg/L.The 72-hour ErC50 was 1.07 mg/L and 95% confidence limit was 1.02-1.12 mg/L.For the initial biomass (0.5 ×104 ind/ml) in the test cultures, the 72-hour EyC50 of 3, 5-dichlorophenol to Scenedesmus obliquus was 1.68 mg/L and 95% confidence limit was 1.63-1.72 mg/L.The 72-hour ErC50 was 1.43 mg/L and 95% confidence limit was 1.42-1.44 mg/L.For the initial biomass (1.0 ×104 ind/ml) in the test cultures, the 72-hour EyC50 of 3, 5-dichlorophenol to Scenedesmus obliquus was 1.55 mg/L and 95% confidence limit was 1.51-1.60 mg/L.The 72-hour ErC50 was 1.39 mg/L and 95% confidence limit was 1.38-1.40 mg/L.[Conclusion] Potassium bichromate and 3,5-dichlorophenol can be used as reference compound on evaluating sensitivity of green alga.%[目的]研究重铬酸钾和3,5-二氯苯酚对羊角月牙藻和斜生栅藻的毒性效应.[方法]以羊角月牙藻和斜生栅藻为试验绿藻,进行了重铬酸钾和3,5-二氯苯酚为参比物的72 h毒性试验.[结果]重铬酸钾对羊角月牙藻的72 h EyC50为1.02 mg/L,95%置信区间为0.98~1.06 mg/L;EryC50为2.48 mg/L,95%置信区间为2.25~2.74 mg/L.3,5-二氯苯酚对羊角月牙藻的72 h EyC50为1.12 mg/L,95%置信区间为1.08~1.16 mg/L;EryC50为2.41 mg/L,95%置信区间为2.22~2.62 mg/L.重铬酸钾对斜生栅藻的72 h EyC50为0.43 mg/L,95%置信区间为0.42~0.43 mg/L;EryC50为1.07 mg/L,95%置信区间为1.02~1.12 mg/L.添加不同初始藻浓度,初始量为0.5×104个/mL时,3,5-二氯苯酚对斜生栅藻的72 h EyC50为1.68 mg/L,95%置信区间为1.63~1.72 mg/L;EryC50为1.43 mg/L,95%置信区间为1.42~1.44 mg/L.初始量为1.0×104个/mL时,3,5-二氯苯酚对斜生栅藻的72 h EyC50为1.55 mg/L,95%置信区间为1.51~1.60 mg/L;EryC50为1.39 mg/L,95%置信区间为1.38~1.40 mg/L.[结论]重铬酸钾和3,5-二氯苯酚可以作为参比物来评价绿藻的敏感度.