地乌泡多糖的提取及其抗氧化作用

摘要

[Objective]To investigate the extraction technology and antioxidant activity of polysaccharides from Rubus irenaeus Focke.[Meth-od]Using phenol-sulfuric acid method as chromogenic agent,the extraction condition of polysaccharides was studied by orthogonal test with the evaluating criteria of extraction rate of polysaccharides.The antioxidant activity was evaluated by DPPH· and ABTS +· scavenging assay. [Result]The optimal extraction conditions were as follow: extraction temperature 80 ℃, solid-liquid ratio 1:15,extraction time 3 h.Under this extraction conditions, the average extraction content of the polysaccharide from the Rubus irenaeus was 251 μg/g.The polysaccharides possessed obviously antioxidant abilities of scavenging the DPPH· and ABTS +· radicals.[Conclusion]The optimal extraction process is sta-ble and reliable;the polysaccharide of Rubus irenaeus has significant antioxidant activity.This study laid the foundation for the comprehensive utilization of the Rubus irenaeus.%[目的]研究地乌泡多糖的提取工艺及其体外抗氧化活性.[方法]采用苯酚-硫酸法作为显色剂,以地乌泡多糖提取量为评价指标,通过正交试验确定地乌泡多糖最佳提取条件;采用DPPH·和ABTS +·清除法评价其抗氧化活性.[结果]地乌泡多糖的最优提取方案:提取温度80 ℃,料液比1:15,提取时间3 h.在此工艺条件下,地乌泡多糖平均提取量为251 μg/g.地乌泡多糖对2种自由基都显示出明显的清除能力.[结论]优选的提取工艺稳定可靠,地乌泡多糖具有显著的抗氧化活性,该研究为地乌泡药材综合利用奠定了基础.