红蛇果接穗组织培养体系的建立

摘要

[Objective] To establish the tissue culture system of red delicious.[Method] Semi-woody stem segments from two-years red delicious seedlings were chosen as the explants in the early spring to establish the tissue culture system by sterilization,germination,multiplication and rooting process.[Result]The most suitable germination medium was MS +6-BA (1.0 mg/L) + IBA (0.1 mg/L) and the sprout rate can up to 93.33％.Adding PVP (0.5 g/L) and Vc (100 mg/L) can effectively prevent browning.The germinated buds were transferred into MS +6-BA (1.2 ～ 1.6 mg/L) which could bring a highly efficient multiplication and inhibit the vitrification with a multiplication rate of 3.79.The most suitable rooting medium for the apical buds was 1/2MS + IBA(1.0 mg/L) and the rooting rate was 34.17％.[Conclusion] The study provided a technical basis for providing a large number of tissue culture seedling of red delicious and subsequent micrografts.%[目的]建立红蛇果接穗组织培养体系.[方法]于早春取红蛇果接穗两年生苗半木质化茎段作为外植体,通过消毒、萌芽、增殖、生根过程建立组织培养体系.[结果]最适宜的萌芽培养基为MS+6-BA(1.0 mg/L) +IBA(0.1 mg/L),其萌芽率可达93.33％,添加PVP(0.5 g/L)和VC(100 mg/L)可以有效地防止褐化.将萌发后的芽转入MS +6-BA(1.2 ～ 1.6 mg/L)中,可以进行高效增殖的同时抑制玻璃化情况的发生,增殖倍率可以达3.79.最适宜红蛇果接穗顶芽生根的培养基为1/2MS+ IBA(1.0 mg/L),生根率为34.17％.[结论]该研究可为提供大量的红蛇果组培苗以及之后的组培微嫁接提供技术基础.