不同光质对金线莲组培苗叶绿素含量及叶绿素荧光参数的影响

摘要

To research the effect of different light quality and the light intensity treatment on the growth of Anoectochilus roxburghii tissue culture seedling, the LED light source was used as an artificial light source for A.roxburghii in Zhangzhou to subculture. The results showed that red light treatment was benefit to the growth of A.roxburghii height, chlorophyll content, Fv/Fm and Fv/Fo values were smaller. Among them, the shoot high of A.roxburghii was up to 6.26 cm under 70μmol/(m2 · s) red light, but the chlorophyll a, chlorophyll b, total content of chlorophyll, Fv/Fm and Fv/Fo were only 1.19 mg/g, 0.55 mg/g, 1.73 mg/g, 0.572 and 1.34;the effect of green light was not obvious for the indicators of A.roxburghii, and no significant difference between different intensities;there was a promoting effect on accumulation of biomass and chlorophyll and Fv/Fm and Fv/Fo of A.roxburghii under the blue light treatment, but certain inhibitory effect on plant height, among them. Under the blue light treatment of 70 μmol/(m2 · s), the chlorophyll a, chlorophyll b, total content of chlorophyll, fresh weight, dry weight, the ratio of dry weight to fresh weight, Fv/Fm and Fv/Fo were up to 1.47 g, 0.19 g, 0.130, 1.83 mg/g, 0.71 mg/g, 2.54 mg/g, 0.801 and 4.02, respectively, but the shoot high of A.roxburghii was only 4.56 cm. Therefore, the red light was advantageous to the growth of aerial parts of plants, the green light had little impact on plant photosynthesis, and there were certain effect on dwarfed and strong tissue culture seedling under the blue light.%为了研究不同光质光强处理对金线莲组培苗生长的影响，采用不同光质LED作为人工光源对漳州金线莲进行继代培养。结果显示：红光处理有利于株高生长，其叶绿素含量、Fv/Fm和Fv/Fo值均较小，其中70μmol/(m2· s)红光处理时金线莲高度达到6.26 cm，但此时其叶绿素a含量、叶绿素b含量、叶绿素总量、Fv/Fm和Fv/Fo值仅分别为1.19 mg/g、0.55 mg/g、1.73 mg/g、0.572和1.34；绿光对金线莲各项指标均无促进作用，且不同强度处理之间差异不显著；蓝光处理对金线莲组培苗叶片生长和生物量积累均有促进作用，金线莲叶绿素含量、Fv/Fm和Fv/Fo值相对较高，但会抑制株高生长，其中70μmol/(m2· s)蓝光处理时金线莲鲜重、干重、干重/鲜重、叶绿素a含量、叶绿素b含量、叶绿素总量、Fv/Fm和Fv/Fo值分别为1.47 g、0.19 g、0.130、1.83 mg/g、0.71 mg/g、2.54 mg/g、0.801和4.02，但其高度仅为4.56 cm。由此得出，红光有利于植物地上部分生长，但是会造成植株徒长，绿光对植物光合作用影响较小，蓝光对组培苗具有一定的矮化壮苗作用。