新蚜虫疠霉菌丝和孢子阶段的转录组学分析

摘要

新蚜虫疠霉(Pandora neoaph idis)是虫霉门(Entomophthoromycota)中极具代表性的蚜虫专化性病原真菌,分布广泛,可侵染多种蚜虫.适宜条件下可在较短的时期内形成流行病,在蚜虫自然防治中起主导作用.为了系统分析了两个阶段的基因表达和功能相关的转录组信息,本研究通过二代测序平台Illumina Hiseq 2500对新蚜虫疠霉的两种发育形态(菌丝和分生孢子)进行转录组测序.得到90 639个不含N(模糊碱基)的组装片段(contigs)和49 138条单基因簇(unigenes).49 138条单基因簇经基因本体论(Gene Ontology,GO)功能显著性富集分类后归到47种功能分支中,相关的基因功能主要归类至分子功能、细胞组分和生化过程三大类中.根据每百万reads中来自于某基因每千碱基长度的reads数(reads perkilobase per million of mapped reads,RPKM)的表达量算法一共得到3 555个差异表达的基因,其中上调的基因有2 294个,下调的基因有1 261个.利用京都基因及基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)对差异表达基因进行途径显著性富集分析后发现与之相关的代谢路径有22条,其中糖胺聚糖降解和类胡萝卜素生物合成两条途径显著上调.本研究为进一步挖掘和分析与新蚜虫疠霉生长发育相关的功能基因提供了理论依据.%Pandora neoaphidis,one of important Entomophthoromycota fungi,is a representative obligated aphid pathogenic fungi that can infect a variety of aphids and are widely distributed.Under suitable conditions,P.neoaphidis can cause a strong epidemic in the aphids in short term and plays a leading role in biological control of aphids.Here,the RNA-seq data of hyphae and primary conidia were carried out using second-generation sequencing platform Illumina Hiseq 2500,and the differentially expressed genes were assessed systematically.Totally,90 639 contigs and 49 138 unigenes were detected among the RNA-seq data.Results based on Gene Ontology (GO) functional enrichment analysis showed that 49 138 unigenes were annotated to 47 function categories,covering 3 ontologies including molecular function,cellular component and biological processes.Besides,according to reads per kilobase per million of mapped reads(RPKM),a total of 3 555 genes expressed differentially,involving in 2 294 up-regulated genes and 1 261 down-regulated genes.Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway enrichment analysis involved 22 metabolic pathways during the conidia stage,of which carotenoid biosynthesis and glycosaminoglycan degradation were significantly up-regulated against the hyphae stage.These basis data would provide a solid foundation for further excavation and analysis of functional genes related to the growth and development of P.neoaphidis.