p53在食道癌中的表达及与淋巴结转移的关系

摘要

目的：探讨p53在食道癌中的表达及与淋巴结转移的关系。方法收集2011年1月～2013年12月经病理检查确诊的食道鳞状细胞癌根治标本154例，其中高分化鳞癌39例、中分化鳞癌56例、低分化鳞癌59例，经常规取材、脱水、透明、浸蜡、包埋、切片、HE染色后镜下观察淋巴结转移情况；并选取癌组织行免疫组化（ S-P法）检测p53。结果154例病例中，高分化鳞癌p53阳性率41％，中分化鳞癌p53阳性率54.2％，低分化鳞癌p53阳性率67.8％；p53阳性而有淋巴结转移者58例、p53阴性而有淋巴结转移者29例。各组间经χ2检验，高分化与中分化及中分化与低分化比较，χ2值分别为2.4及1.38、P＞0.1，无统计学意义；但高分化与低分化比较，χ2值6.89、P＜0.01，具有统计学意义；p53阳性伴淋巴结转移率明显高于p53阴性病例，经检验，χ2值为7.44，P＜0.01，亦具有统计学意义。结论 p53可作为食道鳞状细胞癌重要的预后参考指标。%Objective To investgae the reliability expression of p 53 in esophageal cancer and relationship between expression of p53 and the lymph node metastasis.Methods Collect pathological examination confirmed 154 cases of e-sophageal squamous cell carcinoma radical specimen of our department between 2011 January and 2013 December.A-mong them, 39 cases of highly differentiated squamous carcinoma , 56 cases of moderate differentiated squamous carcino-ma, 59 cases of poorly differentiated squamous carcinoma , through regular based dehydration、transparent、paraffin em-bedding、sectioning,and microscopically observed after HE staining with lymph node metastasis.The expression of p53 in the selective tumor tissue was detected by immunohistochemistry (S-P methods).Results In the 154 cases, the ex-pression of p53 was 41%in the highly differentiated squamous carcinoma , 54.2% in the moderate differentiated squa-mous carcinoma and 67.8%in the poorly differentiated squamous carcinoma.58 cases with lymph node metastasis were p53 positive, 29 cases with lymph node metastasis were p 53 negative.Each group by X2 test, X2value respectively were 2.4 and 1.38 in comparison of highly differentiated squamous carcinoma vs moderate differentiated squamous carcinoma and moderate differentiated squamous carcinoma vs poorly differentiated squamous carcinoma .The P>0.1 ,there were no statistically significant.But X2 value was 6.89 and P<0.01 in comparison of highly differentiated squamous carcinoma vs poorly differentiated squamous carcinoma , which did differ significantly.The rate of P53 positive associated with lymph node metastasis was significantly higher than the p 53 negative cases.By X2test, X2value was 6.89 and P<0.01, which did differ significantly.Conclusions p53 can be taken as a important indicators of the prognosis of esophageal squamous cell carcinoma.