高氯酸钠急性暴露对斑马鱼胚胎发育及氧化应激的影响

摘要

It was chosen perchlorate as exposure compound ,the zebrafish embryo as a test organism to study the effects of acute toxicity and oxidative stress of perchlorate ( PER) to zebrafish embryos .The acute toxicity was processed by the statistical data of surviving rate ,malformation rate,heart rate and hatching rate for zebrafish eggs/larvae;the effects of oxidative stress was processed by SOD、CAT, MDA and Gpx , and relative mRNA expression levels of genes ( Mn-Sod、Cu-Zn-Sod1 ) .The results indicated that PER induced significantly developmental toxicity to zebrafish embryos ,which demonstrated by high death rates and malformation rate in the high concentrations group .Exposure 2000 mg/L PER induced significantly oxidative stress in embryos /larvae,which demonstrated by the activities of SOD and MDA were significantly increased ( P <0.01 ), whereas Gpx levels were significantly decreased (P<0.01);in the same time,the transcription of genes (Mn-Sod、Cu-Zn-Sod1 ) related to oxidative stress were significantly up-regulation ( P <0.01 ).These results indicated that PER could induce acute developmental toxicity and oxidative stress in zebrafish embryos /larvae.%实验选择高氯酸钠（ PER）作为染毒化合物，将斑马鱼胚胎作为受试生物，研究了高氯酸钠对其的发育过程的急性毒性及氧化应激效应．通过对不同质量浓度高氯酸钠（250、500、1000、1500、2000 mg／L）暴露后胚胎／幼鱼发育指标的统计，考查其急性发育毒性．采用SOD、CAT、MDA以及GSH-px、以及Mn-Sod与Cu／Zn-Sod基因表达的分析，研究了高氯酸钠对斑马鱼胚胎／幼鱼的氧化应激效应．结果显示，PER具有显著地发育毒性，表现为高浓度PER导致胚胎／幼鱼死亡，诱导畸形．同时，2000 mg／L PER暴露显著诱导胚胎／幼鱼产生氧化应激，表现为SOD活性显著增加（P＜0．01），MDA含量显著升高（P＜0．001），Gpx活性显著降低（P＜0．01），以及氧化应激功能基因Mn-Sod与Cu／Zn-Sod表达显著上调（P＜0．01）．综上可见，PER存在急性发育毒性，能够诱导斑马鱼胚胎／幼鱼产生氧化应激效应．