真武汤药效物质基础及温阳利水机制研究

摘要

目的：探讨真武汤温阳利水药效物质基础及作用机制。方法回顾真武汤药物成分测定及药物代谢动力学相关研究文献，将真武汤的研究进展进行归纳总结。结果真武汤水煎液中共鉴定出45种成分，主要为来源于附子、白芍和茯苓的生物碱类、多糖类和萜类等成分；共鉴定出真武汤挥发油中的82种成分，主要来自生姜和白术。真武汤温阳利水的作用机制主要包括兴奋下丘脑-垂体-肾上腺皮质轴、调节下丘脑-垂体-甲状腺轴、降低一氧化氮及内皮素含量、调节凋亡相关基因，抑制细胞凋亡以温心阳，调节水通道蛋白AQP1、降低 AQP2，调节渗透压调定点，平衡水液代谢以达到利水目的。结论真武汤温阳机制包括兴奋下丘脑-垂体-肾上腺、下丘脑-垂体-甲状腺以温肾阳，降低一氧化氮及内皮素，抑制心肌细胞凋亡温心阳，通过调节渗透压、调节水通道蛋白增加尿量以达到利水功效，作用机制尚未完全阐明。%Objective To summarize the condition of Zhen-wu decoction overall complexity,classic decoction of warming yang to promote dieresis. Methods Systematically review the papers on Zhen-Wu decoction complexity and pharmacokineticin the past five years. Resullts In total,45 components in Zhen-wu decoction,mainly Alkaloids,poly-saccharide and Terpene were identified,82 components in essential oil of recipe Zhen-Wu decoction,Zingiber,Rhizo-me Atractylodes Macrocephalae were respectively determined qualitatively and quantitatively. The mechanism of war-ming yang to promote dieresis involved the hypothalamo-pituitary-adrenal axis,hypothalamic-pituitary-thyroid axis, Plasma endothelin,balance the quantity and quality of aquaporin one and two,regulating the osmotic pressure. Conclu-sion HPLC and pharmacokinetics had been a key way to study the key component and mechanism of Zhen-Wu de-coction,the further study may answer the mechanism problem of Zhen-Wu decoction.