Objective To observe the intervention effect and influence of different frequencies electro-nape-acupuncture (ENA) on sleep phase of cortex EEG and the contents of serum hormone in insomnia rats induced by PCPA, to discuss the possible mechanism of promoting sleep quality of ENA. Methods Forty male rats were randomized into normal group, model group, ENA group Ⅰ (stimulus frequency of 2 Hz), ENA group Ⅱ (stimulus frequency of 50 Hz) and ENA group Ⅲ (stimulus frequency of 100 Hz), with 8 rats in each group. Acupoints of Fengchi and Gongxue on both sides were used for the each ENA group. 0.25 mm×25 mm disposable sterile acupuncture needles were inserted 7-10 mm. The same side acupoints were connected with wires of electroacupuncture therapeutic meter and corresponding stimulus frequencies were used for treatment. After intervention for seven consecutive days, sleep EEG of rats were recorded. The contents of serum adrenocorticotropic hormone (ACTH) and cortisol (CORT) were measured by enzyme-linked immunosorbent assay (ELISA) in each group. Results Compared with the normal group, total every stage of sleep time decreased (P < 0.01), and ACTH, CORT contents increased in model group (P <0.01). Compared with the model group, TST, FWS, SWS2 of sleep time in EEG of each ENA group significant increased (P < 0.05), (P < 0.01), SWS1 of sleep time significant increased in ENA Ⅰ and ENA Ⅱ groups (P < 0.01), ACTH and CORT contents in ENA Ⅰ and ENA Ⅱ groups decreased significantly (P < 0.05), (P < 0.01). Compared with other treatment group, total sleeping time increased significantly in ENA group Ⅰ (P < 0.01). Conclusion Different frequency electro-nape-acupuncture all can effectively short wake time of insomnia rats and extend the total time of sleep and the therapeutic effect of stimulus frequency of 2 Hz is superior to that of 50 Hz and 100 Hz. The mechanism of promoting sleep may be related to adjusting contents of serum ACTH and CORT.%目的观察不同频率电项针对PCPA致失眠模型大鼠皮层脑电睡眠时相及血清激素含量的干预作用及影响,探讨电项针改善睡眠的可能作用机制.方法将雄性Wistar大鼠随机分成空白组,模型组,电项针1组(取刺激频率2 Hz)、电项针2组(取刺激频率50 Hz)、电项针3组(取刺激频率100 Hz),每组8只.各电项针组选取双侧"风池""供血"穴治疗,用0.25 mm×25 mm一次性无菌毫针刺入7～10 mm,将电针的导线连于同一侧穴位,同时选取相应的刺激频率,干预7 d后描记睡眠脑电.采用酶联免疫吸附法(ELISA)测定各组血清促肾上腺皮质激素(ACTH)、皮质醇(CORT)的含量.结果与空白组相比,模型组的各期睡眠时间均明显减少(P<0.01),ACTH、CORT含量显著升高(P<0.01);与模型组比较,各电项针治疗组脑电图TST、FWS、SWS2期均明显延长(P<0.05,P<0.01),电项针1组和2组SWS1期极显著增加(P<0.01),电项针1组和2组ACTH、CORT含量显著降低(P<0.05,P<0.01);各治疗组间比较,电项针1组的各期时间显著延长(P<0.01).结论不同频率的电项针均可缩短失眠大鼠的觉醒时间,延长大鼠各期睡眠时间,其中2 Hz电针刺激频率效果最佳,其作用机制可能是通过调节血清中ACTH、CORT含量发挥作用.
展开▼
