sFRP、WIF-1、CD133、CD44在食管癌组织中的表达及其临床意义

摘要

Objective To discuss the relationship between the expressions of sFRP,WIF-1,CD133 and CD44 in esophageal cancer and its clinicopathological features,in order to provide new ideas for the study of esophageal cancer. Methods 40 cases of patients with esophageal cancer who suffered excision from January to August 2013 were selected. All the patients were confirmed with esophageal cancer by postoperative pathology. Expressions of sFRP (Secreted Frizzled Related Protein),WIF-1 (Wnt inhibitory factor-1),CD133 (cluster of differentiation 133) and CD44 (cluster of differentiation 44) were analyzed by RT-PCR,and data anal-ysis were made by SPSS 14.0 statistical software with statistical method χ2 test. Results Positive expression rates of sFRP genes, WIF-1 gene,CD44 gene and CD133 gene in esophageal cancer tissues were 32.50%,42.50%,60.00%,72.50%,respectively,while in adjacent tissues were65.00%,70.00%,37.50%,47.50%,respectively. Expressions of CD44 and CD133 gene were higher in esophageal cancer tissues than those in adjacent tissues(P<0.05),in contrast,expressions of sFRP genes and WIF-1 gene were low-er in esophageal cancer tissues than those in adjacent tissues (P<0.05),and the differences were statistically significant.Conclu-sion Expressions of sFRP and WIF-1 in esophageal cancer tissue were lower than those in adjacent tissues,while expressions of CD44 and CD133 in esophageal cancer tissue were higher than those in adjacent tissues. Low expressions of sFRP and WIF-1 genes and high expressions of CD44 and CD133 genes may be associated with the occurrence and development of esophageal can-cer.%目的：探讨sFRP、WIF-1、CD133、CD44在食管癌中的表达与临床病理特征之间的关系，为食管癌的研究提供新思路。方法选取2013年1月-2013年8月经手术切除的食管癌标本40例，术后病理均证实为食管癌患者，采用RT-PCR方法分析sFRP（分泌型卷曲相关蛋白）、WIF-1（Wnt抑制因子-1）、CD133（白细胞分化抗原分化群第133号）、CD44（白细胞分化抗原分化群第44号）的表达情况，应用SPSS 14.0统计软件进行数据分析，采用χ2检验统计方法。结果 sFRP、WIF-1、CD44、CD133基因在食管癌组织阳性表达率为32.50%，42.50%，60.00%，72.50%，在癌旁组织阳性表达率为65.00%，70.00%，37.50%，47.50%。 CD44、CD133基因在食管癌组织中表达水平高于癌旁组织（P<0.05），sFRP、WIF-1基因则在食管癌组织中表达水平低于癌旁组织（P<0.05），差异有统计学意义。结论sFRP、WIF-1在食管癌组织中表达低于癌旁组织，CD44、CD133在食管癌组织中表达高于癌旁组织。 sFRP、WIF-1基因低表达及CD44、CD133基因高表达可能与食管癌的发生、发展有关。