杜仲叶总提取物治疗去势大鼠骨质疏松症的实验研究

摘要

Objective Study of adjusting effect of total extraction in Eucommia ulmoides oliv leaf on bone metabolism in os-teoporosis (OP) model animal’s OP and its mechanism. Methods Thirty-two SD female rats at the age of 4 months were randomly divided into 4 groups:normal control group (gavage with physiological saline),ovariectomized group (OP group,gavage with physio-logical saline),estrogen group (gavage with estrogen),total extraction in Eucommia ulmoides oliv. Leaf group (DZ group,gavage with total extraction of Eucommia ulmoides oliv. leaf). At the end of 3-month treatment,blood calcium (Ca2+),blood phosphor (P3+), and alkaline phosphates (ALP) were tested by orbital blood collection. Thon femur and tibia of 4-group animals were taken out. Bone mineral density (BMD) of them was determined using dual-energy X-ray absorptiometry. Results Compared with OP group, blood calcium (Ca2+),blood phosphor (P3+),and alkaline phosphatase (ALP) in DZ group were decreased significantly (P<0.05);bone mineral density of femurs and tibia in DZ group were remarkably increased (P<0.05). Conclusion Total extraction in Eu-commia ulmoides oliv. leaf can improve bone metabolism of OP animals,increase BMD,prevent and treat osteoporosis effectively.%目的：研究杜仲叶总提取物对去势大鼠骨质疏松（osteoporosis，OP）模型的骨代谢调节作用机制。方法4月龄雌性SD大鼠32只，随机分成正常对照组（Control组，生理盐水灌胃）、去卵巢对照组（OP组，生理盐水灌胃）、雌二醇组（Estrogen组，雌二醇水溶液灌胃）、杜仲叶总提取物组（DZ组，杜仲叶总提物水溶液灌胃）。各组治疗3个月后，通过眼眶采血，测定血清中Ca2+、P3+和ALPase活力。并取大鼠的股骨和胫骨，用双能X线骨密度仪测定骨密度。结果血清中Ca2+、P3+和ALPase活力，DZ组与OP组比较，均有显著差异(P<0.05)；股骨和胫骨BMD，DZ组与OP组比较，有显著差异(P<0.05）。结论杜仲叶总提取物可以改善OP模型动物骨代谢，增加骨质疏松模型动物的骨密度，减少骨破坏，加强骨稳定，有效防治骨质疏松症。