婴幼儿血管瘤与人巨细胞病毒感染相关性的初步研究

摘要

Objective To investigate correlation of the human cytomegalovirus (HCMV) infection and infantile hemangioma (IH). Methods Pathology staining (HE staining) were screened 30 cases of infantile hemangioma (IH) for the experimental group and 30 cases of tumor adjacent tissues as the control group;and were pedestrians CMV pp65 protein immunohistochemistry,im-munofluorescence and half quantitative PCR (RT-PCR) test. Immunofluorescence pp65 expression. Results ⑴IH organization:pp65 protein in all tumor tissues(30 cases),only the expression,the positive rate of 10%in 3 cases,while in tumor and normal tis-sues,30 cases were negative,the protein differences in expression levels was not statistically significant (P>0.05),it showed that pp65 not directly involved IH HCMV formation caused. ⑵RT-PCR results showed pp65mRNA gene in tumor tissues,30 cases of which 20 cases,the positive rate of 66.7%,while in tumor and normal tissues,30 cases of positive samples but only one case,that both differences in expression levels was statistically significant(P<0.05),it showed that pp65 by pp65mRNA regulation may partic-ipate in IH formation caused by HCMV. Conclusion The study shows that the development of HCMV and IH may be relevant, whether or not they have a close relation ship and the specific mechanism needs further study.%目的：初步探讨人巨细胞病毒（HCMV）感染与婴幼儿血管瘤（IH）发病的相关性。方法使用病理染色（HE染色方法）分别筛选出30例婴幼儿血管瘤（IH）为实验组和30例瘤旁组织为对照组；并分别行人巨细胞病毒pp65蛋白免疫组化、免疫荧光以及半定量PCR（RT-PCR）检测。结果⑴IH组织免疫荧光pp65表达：pp65蛋白在所有瘤体组织中（30例），只有3例表达阳性，阳性率10%，而在瘤旁组织中，30例均阴性，即该蛋白表达水平的差异没有统计学意义（P>0.05），表明pp65并未直接参与HCMV导致的IH形成。⑵RT-PCR结果显示：pp65mRNA基因在瘤体组织中，30例当中有20例阳性，阳性率达到66.7%，而在瘤旁组织中，30例标本阳性却仅1例，即二者表达水平的差异有统计学意义（P<0.05），表明pp65通过pp65mRNA可能调控参与了HCMV导致的IH形成。结论研究表明HCMV与IH的发生发展可能具有相关性，但是否密切相关及具体机制如何，有待于进一步研究。