低浓度利福平对鱼藤酮介导的帕金森病细胞模型SH-SY5Y细胞的保护作用

摘要

目的 探讨低浓度利福平对鱼藤酮诱导的人神经母细胞瘤细胞株(SH-SY5Y)细胞的影响.方法 鱼藤酮损伤SH-SY5Y细胞建立帕金森病细胞模型,光学显微镜下观察细胞形态,MTT法检测细胞活性,流式细胞术检测活性氧(ROS)和细胞凋亡率,RT-PCR法检测α-突触核蛋白mRNA.结果 经利福平1、10、100 nmol/L预处理后,三个利福平组细胞ROS含量、α-突触核蛋白mRNA以及细胞凋亡率均低于鱼藤酮损伤组(P＜0.01).结论 利福平具有抗氧化作用,能降低细胞α-突触核蛋白mRNA水平,并对细胞有保护作用.%Objective To explore the effects of low concentrations of rtfampicin on human neuroblastoma(SH-SY5Y) cells. Methods The cells model of Parkinson's disease was established by damaging SH-SY5Y cells with rotenone in vitro. Cell morphology was observed by microscope. Cell viability was measured using MTT assay. Intracellular reactive oxygen species (ROS) and apoptosis rate were measured by flow cytometry. Ot-Synuclein mRNA was measured with RT-PCR. Results Compared with rotenone injury control group, ROS, ot-synuclein mRNA and apoptosis rate in the groups of pretreated with different concentrations(1,10,100 nmol/L) of rifampicin were significantly decreasedC P<0. 01). Conclusion Rifampicin has the effects of anti-oxidative stress, reducing the ct-synuclein mRNA level,and protecting SH-SY5Y cells from apoptosis.