生存素、FHIT、VEGF-C在胃腺癌中的表达及相关性

摘要

目的 探讨生存素(survivin)、脆性组氨酸三联体(FHIT)、血管内皮生长因子C(VEGF-C)在胃腺癌中的表达及其相关性.方法 用半定量RT-PCR检测43例胃腺癌及对应癌旁、远癌正常组织中生存素、FHIT、VEGF-C mRNA的表达.结果 胃腺癌组织中生存素、VFGGF-C的mRNA表达水平明显高于癌旁和远癌正常组织,FHIT mRNA表达明显低于癌旁和远癌正常组织(P＜0.05).生存素、VEGF-CmRNA在胃腺癌中的表达均与肿瘤的分化、分期、浸润、淋巴结转移有关(P＜0.05);FHIT mRNA在胃腺癌中的表达与肿瘤直径、分期、浸润、淋巴结转移有关(P＜0.05).胃腺癌中生存素与VEGF-C的mRNA表达呈正相关(P＜0.05),生存素与FHIT的mRNA表达呈负相关(P＜0.05).结论 生存素、VEGF-C表达增强与FHIT表达缺失共同促进胃癌的发生、发展.%Objective To investigate the expressions and correlation of survivin, fragile histidine triad(FHIT) and vascular endothelial growth factor C(VEGF-C) in gastric adenocarcinoma.Methods The expressions of survivin, FHIT and VEGF-C mRNA were detected by semi-quantitative RT-PCR in cancer tissues, tumor-adjacent normal tissues and distal normal tissues of 43 gastric adenocarcinoma cases. Results Compared with tumor-adjacent normal tissues and distal normal tissues,the expressions of survivin and VEGF-C mRNA increased, while FHIT mRNA decreased,significantly in cancer tissues (P＜ 0. 05 ). The expressions of survivin and VEGF-C in gastric adenocarcinoma were related with tumor differentiation, stage, infiltrating and lymph node metastasis (P＜0.05). The expression of FHIT in gastric adenocarcinoma was related with tumor diameter,stage,infiltrating and lymph node metastasis(P＜0. 05). The expression of survivin mRNA was positively correlated with VEGF-C mRNA (r=0.589, P＜0.05), while negatively correlated with FHIT mRNA (r=-0.453, P＜0.05). Conclusion Higher expressions of survivin and VEGF-C mRNA and lower expression of FHIT mRNA collaboratively promote occurrence and development of gastric adenocarcinoma.