汉防己甲素对TGF-β1诱导L929细胞活化和分泌FN、α-SMA的影响

摘要

Objective To invesgate the effects of tetrandine on TGF-β1-induced activation and secretion of fibronectin(FN) and α-SMA of L929 cells. Methods L929 cells(embroyo lung fibroblast) cultured in vitro were treated with TGF-β1 and tetrandine. The expressions of FN 及 α-SMA mRNA and protein were detected and the morphologic changes were observed. Results That TGF-β1 induced L929 cells for 72 hours upregulated the expressions of FN 及 α-SMA mRNA and protein, which were inhibited by adding tetrandine. Conclusion Tetrandine can inhibit TGF-β1-induced activation and secretion of L929 cells.%目的 研究汉防己甲素对转化生长因子β1 (TGF-β1)诱导的L929细胞活化和分泌纤维连接蛋白(FN)、α-平滑肌肌动蛋白(α-SMA)的作用.方法 体外培养L929细胞,分别以TGF-β1和汉防己甲素作用,观察细胞形态变化,测定FN及α-SMA mRNA、蛋白质表达水平.结果 TGF-β1诱导L929细胞72 h,能显著上调FN及α-SMA mRNA、蛋白质表达；加入汉防己甲素后能显著抑制FN和α-SMA的表达.结论 汉防己甲素能够抑制TGF-β1诱导L929细胞活化和分泌细胞外基质的作用.