CD137L在肺癌细胞株的表达及其生物学功能

摘要

Objective To investigate the expression of CD137 ligand(CD137L) in human lung cancer cell lines and their biological f unction. Methods The mRNA and protein expressions of human CD137L in lung cancer cell lines A549,H460,A2,SK-MES-l and SPC-A1 were detected by RT-PCR and flow cytometry,respectively. A2 cells were divided into three groups of A(coated with PBS).B (coated with lgGl-FC) and C(coated with CD137-FC) , and the cell proliferation and apoptosis were measured by cell counting kit-8 and Annexin V/PI,respectively. Results CD137L mRNA and protein were widely expressed in lung cancer cell lines. The cell proliferation in group C was obviously higher than that in groups of A and B(P<0. 05) .whereas there was no significant difference in cell apoptosis among groups of A.B and C(P>0. 05). Conclusion CD137L is widely expressed in various kinds of lung cancer cell lines. CD137L-mediated reverse signal can promote cell proliferation of lung cancer and may play an important role in the development and progression of lung cancer.%目的 研究人CD137配体(CD137L)在人肺癌细胞株的表达及其生物学功能.方法 采用RT-PCR及流式细胞术检测人CD137L在肺癌细胞株A549、H460、A2、SK-MES-1、SPC-A1的表达水平.用细胞计数试剂盒8检测A2细胞在PBS(A组)、IgG1-FC(B组)或CD137-FC(C组)包被处理后的增殖情况,并用Annexin V/PI双染方法检测其凋亡率.结果 CD137L mRNA及蛋白在多种肺癌细胞株上均有表达.C组细胞增殖较A、B组明显增加(P＜0.05),但三组间细胞凋亡率差异均无统计学意义(P＞0.05).结论 CD137L在各种肺癌细胞株上均有表达,CD137L介导的逆向信号能促进肺癌细胞增殖,可能在肺癌发生、进展中起重要作用.