人类多重剪接RNA结合蛋白类基因的克隆和分析

摘要

Objective To assemble and clone human RNA binding protein with multiple splicing 2(RBPMS2) gene by homologous sequence aligning. Methods Human RBPMS2 gene was taken as an information probe for searching the coding sequece in GeneBank database by homologous sequence aligning. Then the highly homologous expressed sequence tag(EST) was assembled to the contig using VECTOR NTI software. The chromosome localization,domain and expression pattern of RBPMS2 were analyzed by UCSC Genome Blat Server, SMART and Unigene database, respectively. Results A novel cDNA sequence of human RBPMS2 gene was cloned, which contained complete open reading frame(ORF). RBPMS2 gene encoded a 209 amino acides protein with MW 22. 5 KDa and pi 8.63. SMART analysis showed that RBPMS2 protein included a RNA recognition motif (RRM) domain. RBPMS2 was mapping on the fifteenth chromosome, located at 15q22. 31, and consisted of seven exons and six introns. Unigene database presented that RBPMS2 was widely expressed in ovotid,zygote,blastula,embryo at different developmental stages, bladder and kidney. Conclusion A novel human RBPMS2 gene has been cloned and analyzed.%目的 通过同源筛选的方法拼接并克隆人类多重剪接RNA结合蛋白2(RBPMS2)基因.方法 采用同源筛选策略,以人RBPMS2基因作为信息探针,在GeneBank数据库中进行分析,将获得的高度同源的表达序列标签(EST)用VECTOR NTI软件拼接成重叠群.通过UCSCGenome Blat Server分析,确定RBPMS2基因的染色体定位,SMART网上分析工具进行结构域预测,Unigene数据库进行表达谱分析.结果 电子克隆到人类RBPMS2新基因cDNA序列,含完整的开放阅读框(ORF).RBPMS2基因编码的多肽由209个氨基酸组成,分子量22.5 KDa,等电点8.63.SMART分析显示RBPMS2蛋白包含1个RNA识别模体(RRM)结构域.RBPMS2基因位于第15号染色体,定位在15q22.31,由7个外显子和6个内含子组成.电子表达谱分析显示RBPMS2在卵细胞、受精卵、囊胚、不同发育阶段的胚胎、膀胱、肾脏等组织中高表达.结论 分析并克隆到了一个新的人类RBPMS2基因.