Objective To investigate the evaluation and clinical significance of CD 4+ CD45RO+ /CD45RA+CD127+ T cells in the course of AIDS patients .Methods The expressions of virgin and memory CD4+ and CD8+ T cell surface CD127 molecules were detected by flow cytometry in AIDS patients undergoing highly active anti-retroviral therapy (HAART ) ,who were divided into three groups of A (with the absolute number of CD4+ T cells less than 350 pieces/mm3 ) ,B (350-800 pieces/mm3 ) and C(more than 800 pieces/mm3 ) .The expressions of CD19 ,CD8/38 and CD56 on the surface molecules of B lymphocytes and natural killer cells were detected by flow cytometry . Meanwhile ,HIV virus in plasma was detected by fluorescent quantitation PCR in AIDS patients with stable status and viral load less than 50 copies/ml .The results were compared among four groups . Results In 39 patients with effective HAART ,the CD4+ CD45RO+ CD127+ T cell count was positively correlated with CD4+ ,CD4+ CD45RA+ and CD4+ CD45RO+ CD127+ ( r=0 .649 ,0.539 and 0.997 ,P<0 .05 ) ,so did the CD4+CD45RA+CD127+ and CD4+ ,CD4+CD45RO+ and CD4+CD45RA+ (r=0.550 ,0.497 and 0.992 ,P<0 .05) .The significant differences existed in CD4+CD45RA+ , CD4+CD45RO+ ,CD4+ CD45RA+ CD127+ and CD4+CD45RO+CD127+ marked molicule CD19 among four groups(P<0 .05) .Conclusion The CD4+ T cell groups take a very important role in against virus copying in the process of HIV immune reconstitution ,in which the CD4+ CD45RO+ CD127+ T cell play a more important role in the patients with AIDS .%目的 探讨CD4+CD45RO+ /CD45RA+CD127+ T细胞在AIDS病程中的评价及其临床意义.方法 采用流式细胞术检测经高效抗逆转录病毒治疗(HAART )的 AIDS的CD4+ 以及CD8+ T细胞表面CD127分子的表达 ,以CD4+ T细胞绝对数分为三组 :A组 ,<350个/mm3 ;B组 , 350~800个/mm3 ;C组 ,>800个/mm3 .同时用荧光定量PCR技术检测AIDS患者血浆中 HIV (病毒载量小于50 copies/ml ,病情稳定者) ,用流式细胞术检测上述患者外周血B淋巴细胞及自然杀伤细胞表面分子CD19、CD8/38和CD56的表达 ,比较组间差异.结果 在经 HAART 有效的39例AIDS中 ,其CD4+CD45RO+CD127+ T细胞数与患者CD4+ 、CD4+CD45RA+和CD4+CD45RO+细胞数呈显著正相关(相关系数分别为0. 649、0. 539和 0. 977 ,P<0 .05 );CD4+ CD45RA+ CD127+ 与患者CD4+ 、CD4+CD45RO+ 和 CD4+ CD45RA+ 有显著相关性(相关系数分别为 0.550、0.497和0.992 , P<0 .05).CD4+CD45RA+ 、CD4+CD45RO+ 、CD4+ CD45RA+ CD127+和CD4+ CD45RO+ CD127+标记CD19分子组间比较均有统计学差异(P<0 .05).结论 在AIDS免疫重建过程中 ,CD4+ T细胞亚群在对抗病毒的复制起了主要作用 ,CD4+CD45RO+CD127+ T调节细胞群对于AIDS患者的免疫重建作用比较重要.
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