生物膜载体对富营养化水体细菌群落结构的影响

摘要

利用主要成分为秸秆处理物(ZT2)、颗粒状活性炭(ZT3)和纤维碳膜(ZT4)作为生物膜载体材料对富营养化水体进行生物脱氮.室内模拟试验结果表明,3个处理对富营养化水体全氮的去除率达到了40％以上.ZT2、ZT3和ZT4生物膜表面细菌比对照提高了2个数量级,硝化细菌和反硝化细菌数量也提高10倍左右.PCRDGGE检测结果显示,添加生物膜载体材料能够增加水体中细菌群落的多样性.ZT2、ZT3和ZT4处理的ShannonWiener指数由2.838分别提高到3.483、3.498和3.458,丰度由19提高到了36、41和35.%The three different materials as bio-film carriers were applied into biological denitrogenation of eutrophic water. The materials were ZT2 (straw), ZT3 ( granular activated carbon) and ZT4 (fiber carbon film). The results of simulation experiments (in greenhouse) showed that total nitrogen removal rates by ZT2, ZT3 and ZT4 reached up to over 40%. Compared with control, the bacterial count on bio-film surfaces of ZT2, ZT3 and ZT4 were enhanced by two orders of magnitude, and the nitrifying or denitrifying bacterial count were also enhanced by approximate 10 times. Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) result indicated that the bio-film carriers could improve the diversity of bacterial community in eutrophic water. Shannon-Wiener indexes of ZT2, ZT3 and ZT4 treatments increased from 2. 838 to 3.483, 3.498 and 3.458 respectively, and richnesses also increased from 19 to 36, 41 and 35.