斜纹夜蛾核型多角体病毒安徽和县株系细胞凋亡抑制相关基因的克隆与遗传变异

摘要

为分析核型多角体病毒( NPV)分离株内细胞凋亡抑制相关基因的多态性,从安徽和县采集感染病毒的斜纹夜蛾病虫,分离出斜纹夜蛾核型多角体病毒(Spodoptera litura nuc1eopo1yhedrovirus, Sp1tNPV)15个活体克隆株系,研究其细胞凋亡抑制基因( IAP)和P49基因多态性。发现15个Sp1tNPV安徽和县克隆株系中的IAP基因序列均无差异,且与已报道的Sp1tNPV中国G2株的同源性达100%；Sp1tNPV各克隆株系中的P49基因序列也无差异,且与Sp1tNPV中国G2株的P49基因同源性达99%。表明Sp1tNPV安徽和县株系IAP和P49基因均较为保守,没有表现出遗传变异。%The po1ymorphism of genes re1ated to the inhibition of apoptosis protein ( IAP) in nuc1eopo1yhedrovirus from Anhui Hexian iso1ate of Spodoptera litura( Sp1tNPV) was identified and the genetic variations of IAP gene and P49 gene. There was no genetic differentiation of IAP gene or P49 gene in 15 c1ones of Sp1tNPV Hexian iso1ate was found. The gene sequence a1ignment showed that the sequence of IAP gene of Sp1tNPV Hexian iso1ate shared 100% identity with that of Sp1tNPV G2 iso1ate, and the sequence of P49 gene of Sp1tNPV Hexian iso1ate shared 99% identity with that of Sp1tNPV G2 iso1ate. The resu1ts indicated that the IAP and P49 genes were high1y conservative, and genetic differentiation of the two genes was not found in the Sp1tNPV Hexian iso1ate.