基于重组自交系群体的水稻粒形QTL定位

摘要

以云南省地方籼稻品种扎西玛与江苏省著名优质粳稻品种南粳46杂交获得的水稻重组自交系群体为研究对象,考察了各株系籽粒长度、籽粒宽度、籽粒长宽比和籽粒厚度4个性状,结合利用202个SSR分子标记构建的该群体分子连锁图谱对控制水稻粒形相关性状的QTL进行分析.共检测到17个加性QTL.其中粒长相关的3个,增效等位基因均来自扎西玛;粒宽相关的3个,增效等位基因均来自南粳46;籽粒长宽比相关的6个,其中5个QTL增效等位基因来自扎西玛;粒厚相关的5个,其中4个增效等位基因来自南粳46.经与前人研究结果比对,共发现2个粒形新位点,分别控制粒长和粒宽.%In this research, recombinant inbred lines(RILs) derived from Zhaxima, a landrace indica variety of Yunnan province and Nanjing 46, an elite japonica variety with superior grain quality of Jiangsu province were used.A molecular linkage map consisting of 202 SSR markers was constructed.Grain shape related traits including grain length, grain width, grain length-width ratio and grain thickness were investigated in the RIL population.Combined with the linkage map, a total of 17 additive QTL affecting grain shape were detected, in which three controlling grain length, all positive alleles from Zhaxima;three controlling grain width, all positive alleles from Nanjing 46;six controlling grain length-width ratio,of which five positive alleles from Zhaxima;five controlling grain thickness, of which four positive alleles from Nanjing 46.Compared with the previous research results, two QTLs, controlling grain length and grain width respectively, were found in this study.