目的 探讨短时受精精卵共孵育时间对受精率的影响.方法 170例行体外受精-胚胎移植( IVF-ET)患者常规促排卵,依据精卵共孵育时间分为短时受精组(A组:57例,精卵共孵育2～3h；B组:58例,精卵共孵育4～5h)和常规受精组(C组:55例,精卵共孵育18 h );之后将卵子转移至新鲜培养基中.比较各组的受精率、卵裂率、优胚率、临床妊娠率、胚胎种植率.结果 三组受精率分别为63.01％、71.43％和78.72％,各组间的差异有统计学意义(P＜0.05)；C组的卵裂率、优胚率均低于A组和B组；C组与A组的卵裂率[97.05％ vs 99.25％]、C组与B组的优胚率[37.14％vs 44.42％]之间的差异有统计学意义(P＜0.05)；各组间临床妊娠率、胚胎种植率差异无统计学意义(P＞ 0.05).结论 短时受精有助于提高卵裂率、优胚率,精卵共孵育时间4～5h的受精率较2～3h高.%Objective To investigate effects of duration of sperm and egg co-incubation on the fertility rate in short time insemination.Methods 170 women undergoing in vitro fertilization - embryo transfer ( IVF-ET ) were divided into short-time insemination group ( group 1∶57 women,co-incubation for 2 h to 3 h; group 2:58 women,incubation for 4 h to 5 h ) and routine insemination group ( group 3∶55 women,co-incubation for 18 h ) based on duration of sperm and egg co-incubation.Then the oocytes were transferred to the fresh medium.The rates of fertility,cleavage,high-quality embryo,pregnancy, and implantation were compared among the groups.Results The fertility rate was 63.01％,71.43％,and 78.72％ in each group,with a significant difference ( P＜ 0.05 ).The rates of cleavage and high-quality embryo were lower in group 3 than in groups 1 and 2.There were significant differences in the cleavage rate between group 3 and group 1 ( 97.05％ vs.99.25％ ) and in the high-quality embryo rate between group 3 and group 2 ( 37.14％ vs.44.42％ ) ( P＜ 0.05 ).There were no significant differences in the rates of pregnancy and implantation among the three groups ( P ＞ 0.05 ).Conclusions Short-time insemination is beneficial for elevating the rates of the cleavage and high-quality embryo.The fertility rate of sperm and egg co-incubation for 4 h to 5 h is higher than that of co-incubation for 2 h to 3 h.
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