血清AMA-M2亚型、调节性T细胞与IL在PBC患者中的表达及临床意义

摘要

目的 研究原发性胆汁性肝硬化(PBC)患者血清抗线粒体抗体亚型M2(autimitochondria antibody typeⅡ,AMA-M2)、调节性T细胞和相关白介素(IL)的表达及相关性,以探讨其诊断价值.方法 分别收集PBC确诊患者组(42例)、疾病对照组(36例)和健康对照组(50例)的血清样本,采用免疫印迹法检测AMA-M2;流式细胞术检测CD4±CD25+调节性T细胞和CD4+、CD8+、CD4+、CD8+水平;IL-2、IL-4、IL-6、IL-8、IL-10采用ELISA定量方法测定.结果 PBC组、健康对照组和疾病对照组AMA-M2阳性率差异有统计学意义(P＜0.05).与AMA-M2阴性组比较,阳性组CD4+、CD4+/CD8+分别增加11.57％和72.79％,CD4+CD25+、CD8+水平分别降低61.50％和35.97％,IL-2、IL-4、IL-6、IL-8、IL-10分别升高189.65％、24.29％、2184.54％、230.33％和1281.37％,且差异均有统计学意义(P＜0.05);但与弱阳性组相比,阳性组血清中只有CD4+、CD8+、IL-4、IL-6、IL-8、IL-10差异有统计学意义(P＜0.05).结论 PBC患者免疫功能异常与IL-2、IL-6、IL-8、IL-10高水平表达相关,血清AMA-M2阳性检测、外周血调节性T细胞比例及细胞因子浓度的检测可为临床诊断PBC提供依据.%Objective To study the expressions and correlation of serum anti-mitochondrial M2 antibodies (auti-mitochondria antibody type Ⅱ,AMA-M2),regulatory T cells,and relevant interleukin (IL) in patients with primary biliary cirrhosis (PBC),and to explore the diagnostic value.Methods The serum samples of the PBC diagnosed patients group (n=42),disease control group (n=36),and healthy controls (n=50) were collected respectively.Western Blot Method was used to detect AMA-M2 and the CD4+CD25+ regulatory T cells and the levels of CD4+,CD8+,CD4+/CD8+ were examined by the Flow Cytometry.The ELISA quantitative method was used to determinate IL-4,IL-6,IL-2,IL-8,and IL-10.Results There were statistical differences in the positive rate of AMA-M2 hetween the PBC group,the healthy controls,and the disease control group (P ＜ 0.05).Compared with the AMA-M2 negative group,the levels of CD4+ and CD4+/CD8+ were increased by 11.57％ and 72.79％ respectively,the CD4+CD25+ and CD8+ levels were decreased by 61.50％ and 35.97％ respectively,IL-2,IL-4,IL-6,IL-8,and IL-10 were increased by 189.65％,24.29％,2184.54％,230.33％,and 1281.37％ respectively in the positive group,with statistical differences (P ＜ 0.05).But in comparison with the weak positive group,there were statistical differences in only CD4+/CD8+,IL-4,IL-6,IL-8,and IL-10 between the weak positive and positive groups (P ＜ 0.05).Conclusions The immune dysfunction of the patients with PBC correlates with high expression levels of IL-2,IL-6,IL-8,IL-10.Positive serum AMA-M2 detection,the proportion of regulatory T cell in peripheral blood,and the detection of cytokine concentration can provide references for clinicaly diagnosing PBC.