辛弗林对大鼠离体小肠收缩性的调节特征

摘要

Objective To investigate the effect of Ca2+ concentration on contractility of rat's isolated intestine,and the effect of synephrine on contractility of rat's isolated intestine under the most suitable Ca2+ concentration.Methods Isolated rat intestine was selected in the assay to test the changeextent of contractile amplitude using Krebs' solution in different Ca2+ concentration,observed the effect of synephrine on contractility of isolated rat intestine in different Ca2+ concentration.Result The contractile amplitude decreased most significantly after adding synephrine when the concentration of Ca2+ was 5 mmol/L.Conclusion 5 mmol/L Ca2+ is the most suitable concentration to study inhibitory effect of a drug on isolated rat intestine in the corresponding assay condition.%目的 考察不同Ca2+浓度对大鼠离体小肠收缩性的影响;选择最佳Ca2+浓度,探讨在该条件下辛弗林对离体肠收缩性的影响.方法 本研究以大鼠的离体小肠为模型,通过不同浓度的Ca2+克氏液,使小肠处于不同收缩状态;考察在不同Ca2+浓度条件下,辛弗林对离体小肠的收缩的影响.结果通过辛弗林对不同浓度的Ca2+条件下的作用比较,结果 提示在5 mmol/L的Ca2+情况下,加入辛弗林后,肠平滑肌收缩幅度降低最明显.结论结果提示5 mmol/L Ca2+的浓度是在本实验条件下,用于研究对肠道平滑肌收缩具有抑制作用药物的最佳条件.