HPLC法测定阿司匹林微囊中阿司匹林的含量

摘要

目的 建立检测阿司匹林微囊中阿司匹林含量的高效液相色谱分析法,为定量测定阿司匹林微囊中阿司匹林的含量提供检测手段.方法 用十八烷基硅烷键合硅胶为填充剂,以乙腈:0.1％磷酸为流动相(梯度洗脱:0min:乙腈:0.1％磷酸=10∶90;1 min:乙腈:0.1％磷酸=30∶70),在波长为276nm时进行检测,理论塔板数按阿司匹林峰计算不低于l 500.结果 阿司匹林和水杨酸分别在10～ 100μ g/ml (r=0.999 1)和4～40μg/ml (r=0.9995)浓度范围内呈良好的线性关系,平均回收率为96.91％,RSD=1.69％ (n=6).结论 本法具有简单、准确、灵敏度高的特点,适用于阿司匹林微囊中阿司匹林含量的测定.%Objective To provide a method for detecting the content of aspirin in aspirin microcapsules using high performance liquid chromatography (HPLC) to determine the content of aspirin in aspirin microcapsules.Methods Octadecyl silane bonded silica was used as filler and acetonitrile:0.1％ phosphoric acid as mobile phase (gradient elution:0 minute:acetonitrile:0.1％ phosphoric acid =10∶90;1 minute:acetonitrile:0.1％ phosphoric acid =30∶70),when the wavelength was 276 nm,the detection was carried out and theoretical plate number was calculated in term of aspirin peak not below 1 500.Results Aspirin had a linear relationship with salicylic acid when the content was 10 ～ 100 μg/ml (r =0.9991) and 4 ～ 40 μg/ml (r =0.9995).The average recovery rate was 96.91％.RSD =1.69％ (n=6).Conclusion This method is simple,accurate,and highly sensitive,so it is suitable for the detection of the content of aspirin in aspirin microcapsules.