腺病毒介导的靶向survivin的siRNA上调乳腺癌MCF-7细胞雌激素受体表达

摘要

目的 探讨腺病毒介导的靶向survivin的小干扰RNA(siRNA)对乳腺癌MCF-7细胞雌激素受体(ER)表达的影响.方法 构建靶向survivin的siRNA腺病毒载体并转染乳腺癌MCF-7细胞,采用Western blot法检测MCF-7细胞转染腺病毒后survivin、ER表达的变化.结果 实验组、阴性组、空白组survivin的表达强度分别为0.09 ±0.04、0.86±0.08、0.82 ±0.17;ER的表达强度分别为1.57 ±0.09、1.16±0.10、1.23±0.01.统计学分析表明,本研究中所构建的靶向survivin的siRNA腺病毒载体可以高效地抑制survivin的表达,抑制survivin的表达可以显著上调乳腺癌MCF-7细胞ER的表达(P＜0.05).结论 在乳腺癌MCF-7细胞中,survivin与ER信号通路之间可能存在着某种相互调节机制,提示靶向survivin的siRNA在ER阳性乳腺癌的内分泌治疗中可能具有潜在的重要意义.%Objective To investigate the effect on ER expression in MCF-7 cell by siRNA against survivin mediated by adenovirus vector.Methods An adenovirus vector of siRNA against survivin was constructed and used to infect MCF-7 cell.The change of expression of survivin and ER was detected by Western Blot.Results The expression strength of survivin are 0.09 ± 0.04、0.86 ± 0.08、0.82 ± 0.17;expression strength of ER are 1.57 ± 0.09、1.16 ± 0.10、1.23 ± 0.01 respectively in the experimental group,negative control group and blank control group.Statistics analysis shows that the adenovirus vector of siRNA against survivin constructed in the study can suppress the expression of survivin significantly,and suppress the expression of survivin can up-regulate the estrogen receptor (ER) expression.Conclusions The results suggest that there may be a certain regulatory mechanism between survivin and ER signal pathway in MCF-7 cell and siRNA against survivin is of important potential value in the endocrine therapy of hormone receptor positive breast cancer.