姜黄素对哮喘小鼠Nrf2及HO-1的含量变化与表达的影响

摘要

目的 了解姜黄素对哮喘小鼠核因子相关因子2(nuclear factorerythroid-2-related factor 2,Nrf2)及血红素氧化合酶(HO-1)的含量变化与表达的影响.方法 将45只Balb/c小鼠随机分为正常对照组、哮喘组及姜黄素干预哮喘小鼠.应用免疫荧光检测各组小鼠肺组织Nrf2及HO-1的表达;应用Western blot方法检测各组小鼠肺组织Nrf2及HO-1的蛋白含量;应用EMSA方法测定各组小鼠肺组织Nrf2与ARE的结合活力.结果 免疫荧光检测显示姜黄素干预组肺组织较哮喘组及正常对照组Nrf2及HO-1的表达均增高;各组小鼠肺组织胞浆内Nrf2的蛋白含量无明显差异(P＞0.05),姜黄素干预组肺组织胞核内Nrf2及HO-1蛋白含量较其余两组明显增高,差异有统计学意义(Nrf2,P＜0.05;HO-1,P＜0.01).姜黄素干预组小鼠肺组织Nrf2-ARE的结合活力明显高于哮喘组及对照组,差异有统计学意义(P＜0.05).结论 姜黄素可以增加哮喘小鼠体内Nrf2及HO-1的蛋白含量,减轻哮喘.%Objective To investigate the effect of the asthmatic mice's contents of nuclear factorerythroid-2-related factor 2,Nrf2 and HO-1 reated with curcumin in lung tissue.Methods All 45 mice were divided into three teams randomly,the normal mice,the asthmatic mice and the curcumin mice group.We tested the expression of Nrf2 and HO-1 of three mice teams in lung by immunolfluorescence technique.We tested the protein contents of Nrf2 and HO-1 of the three teams in the lung tissue by Western blot.We tested the binding activity of Nrff2 and ARE of the three teams in lung by EMSA.Results The expression of Nrf2 and HO-1 in curcumin group was significantly higher than that in asthma group and normal control group by immunolfluorescence technique.There was no sigrnificant difference in content of cytoplasm Nrf2 protein in lung tissue between three groups by western blot(P ＞ 0.05).The content of nuclear Nrf2 and HO-1 protein in the lung tissue of the curcumin group was significantly higher than that in the other two groups,the difference is statistically significant (Nrf2,P ＜0.05;HO-1,P ＜0.01).The binding activity of Nrf2-ARE in lung tissue of curcumin group was significantly higher than that of asthma group and control group,with statistical significance(P ＜ 0.05).Conclusion Curcumin can increase the protein contents of the Nrf2 and HO-1 of mice and enhance the expression of them.