腺病毒载体构建研究进展

摘要

Adenovirus vectors are widely used in many fields such as in vitro and in vivo gene transduction,gene therapy,vaccination and oncolytic treatment.They have high titer,low pathogenicity,high transduction efficiency,wide infected tissue and no host cell genome integration and many other advantages,and are widely used in experimental and clinical research.In recent years,using different methods of experimental improvement of adenoviral vectors in the basic and clinical research has made great development,becoming the most promising aspect of the viral vectors.This article reviews the structure of adenovirus,the development characteristics of adenoviral vectors and the latest improvements,clinical applications and other aspects of research progress.%腺病毒载体广泛用于基因转导、基因治疗、疫苗接种以及溶瘤治疗等多个领域,具有滴度高、致病性低、转导效率高、感染组织广、无宿主细胞基因组整合等诸多优点,在实验和临床研究中被广泛应用.近年来,采用不同方法经过实验改进的腺病毒载体在基础和临床研究中已取得较大的发展,成为目前最具有应用前景的病毒载体之一.该文就腺病毒的结构、腺病毒载体的研发和最新改进、临床应用等多个方面的研究进展进行综述.