上皮-间质转化在蒿甲醚逆转结直肠癌细胞放化疗抵抗中的作用

摘要

Objective:To investigate the effect of epithelial-mesenchymal transition (EMT) on the reversion effect of chemo-radiation resistance of artemether (ARE) in human colorectal cancer HCT116CRR cell line.Methods:The experiment was divided into four groups:1) Normal control group;2) Chemoradiotherapy group;3) ARE combined with chemoradiotherapy group;4) ARE group.RT-PCR and Western blot was used to detect the expressions of mRNAs and proteins of E-Cadherin,N-Cadherin,Vimentin,Snail.Results:RT-PCR and Western blot results:E-cadherin mRNA and protein expression level,chemoradiotherapy group ＜ARE combined with chemoradiotherapy group ＜ Normal control group ＜ ARE group (P＜0.05);N-cadherin,Snail,Vimentin mRNAs and proteins expression level,chemoradiotherapy group ＞ ARE combined with chemoradiotherapy group ＞ Normal control group ＞ Artemether group (P＜0.05).Conclusion:ARE plays a role of reversion to chemo-radiation resistance in human colorectal cancer HCT116CRR cell line by modulating the EMT-related molecular markers.That is to say,artemether can up-regulate the mRNA and protein of E-cadherin and down-regulate the mRNAs and proteins of N-cadherin,vimentin,and snail.%目的:探讨上皮-间质转化(epithelial-mesenchymal transition,EMT)在蒿甲醚(artemether,ARE)对同期放化疗抵抗人结直肠癌细胞HCT116(HCT1 16CRR)的逆转作用中的作用.方法:实验分为四组:1)对照组;2)单纯放化疗组;3)ARE联合放化疗组;4)ARE组.采用Real-time PCR和Westernblot,检测各组EMT相关指标E-cadherin,N-cadherin,vimentin,snail mRNA及其蛋白的表达情况.结果:Real-time PCR和Western blot结果显示E-cadherin mRNA及其蛋白的表达情况,单纯放化疗组＜ARE联合放化疗组＜对照组＜ARE组;N-cadherin,vimentin,snail mRNA及其蛋白的表达情况,单纯放化疗组＞ARE联合放化疗组＞对照组＞ARE组.结论:EMT在ARE逆转同期放化疗抵抗人结直肠癌细胞HCT116CRR细胞系的放化疗抵抗作用中有重要的作用,即上调E-cadherin mRNA及其蛋白的表达,下调N-cadherin,vimentin,snail mRNA及其蛋白的表达.