原花青素的抗炎作用及其作用机制探讨

摘要

目的 研究原花青素的抗炎作用及其作用机制.方法 以二甲苯致炎小鼠耳片肿胀及角叉菜胶诱发大鼠足爪肿胀模型,分别探讨原花青素对小鼠耳片肿胀及大鼠足爪肿胀的抑制作用.用紫外分光光度法测定足爪炎性渗出液中前列腺素E2(prostaglandin E2,PGE2)的含量.WesternBlot法和免疫组化法(immunohistochemistry,IHC)检测大鼠足爪中环氧合酶-2(Cyclooxygenase-2,COX-2)的表达情况.结果 10 mg/kg和20 mg/kg的原花青素抑制二甲苯致炎小鼠耳片肿胀,原花青素5 mg/kg和20.0 mg/kg ip在2～5 h抑制角叉菜胶诱导的大鼠足爪肿胀.原花青素5 mg/kg和20.0 mg/kg ip能抑制大鼠炎症足爪组织PGE2的牛物合成.原花青素5 mg/kg和20.0 mg/kg ip能明显下调COX-2的表达.结论 原花青索具有较强的抗炎作用,其抗炎作用可能与抑制COX-2的表达继而下调PGE2的生物合成有关.%Objective To investigate the anti-inflammatory effect of proanthocyanidins and its mechanisms.Methods Inflammation models such as dimethylbenzene-indueed ear swelling and carrageenan-induced hind paw edema in mice and rats were prepared.The contents of PGE2 in exudate from edema paws of rats were measured by ultraviolet spectrophotography and the protein expression of COX-2 in edema paws of rats by Western-blot and immunohistoehemistry(IHC)assay.Results Pro-anthocyanidins remarkably inhibited the ear edema induced by dimethylbenzene in mice at the dose of10 mg/kg and 20 mg/kg;paw edema of rats induced with carrageenan was significantly inhibited byproanthocyanidins 5,20 mg/kg ip from 2 to 5 h;proanthoeyanidins 5,20 mg/kg ip reduced the contents of PGE2 in exudate from edema paws of rats induced by carrageenan;proanthocyanidins 5,20 mg/kg ip inhibited the protein expression of COX-2.Conclusion Proanthocyanidins has an anti-inflammatory effect in vivo which may be related to inhibition of protein expression of COX-2 and downregutation of PGE2 biosynthesis.