EGCG对体外诱导小鼠Th17细胞的产生及促炎因子调控的影响

摘要

目的 探讨表没食子儿茶素没食子酸酯(EGCG)对体外诱导小鼠Th17细胞的产生及促炎因子白细胞介素(IL)-17和肿瘤抑制因子(TNF)-α的表达和分泌的影响.方法 分离小鼠脾脏CD4+T淋巴细胞,加入刺激体系诱导体外培养的淋巴细胞活化分化成Th17细胞.设立对照组和不同剂量EGCG组(100、200、400 ng/mL),分别培养72 h后,采用流式细胞仪检测Th17细胞.分别采用荧光定量PCR和ELISA法检测培养细胞IL-17、TNF-α的基因表达和蛋白分泌水平.结果 EGCG能够抑制体外诱导的Th17细胞的产生,IL-17和TNF-α的基因表达和蛋白分泌水平均受到显著抑制,且其抑制作用呈剂量依赖性.结论 EGCG可抑制体外诱导的小鼠Th17细胞产生,抑制IL-17和TNF-α的表达和分泌.%Objective To explore the effects of epigallocatechin-3-gallate(EGCG) on proliferation of Th17 cells and the contents of interleukin-17(IL-17) and tumor necrosis factor-α(TNF-α) in vitro.Methods Splenic CD4 + T cells in BALB/c mice were activated and divided into control group(without EGCG) and experiment groups(with 100,200,400 ng/mL EGCG respectively).Each group was incubated for 72 h.Th17 cells were measured by flow cytometry(FCM) method.The levels of IL-17 and TNF-α mRNA were detected by real time-PCR.The levels of IL-17 and TNF-α in supernatant were determined by enzyme-linked immunosorbant assay(ELISA).Results EGCG could inhibit the growth of Th17 cells.In addition, EGCG could also decrease the mRNA and the supernatant protein levels of IL-17 and TNF-α in a dose-dependent manner.Conclusion EGCG can significantly inhibit Th17 cell growth and the expression of IL-17 and TNF-α in vitro.